Considering the glulam beam-column connection form and the number of bolts, monotonic loading test and finite element analysis was carried out on 9 connection specimens in 3 groups to study the rotational performance and failure mode of the connection. The test results revealed that compared with U-shaped connectors, T-shaped connectors can effectively improve the ductility of connections, and the increase in the number of bolts can reduce the initial stiffness and ductility of connections. By theoretical analysis, formulas for calculating the initial stiffness and ultimate moment of connections were deduced. Subsequently, the moment-rotation theoretical model of connections was established based on the formulas, which were validated according to the test data and simulation results. The proposed model can not only improve the current theoretical system of heavy-duty glulam beam-column structure but also provide a theoretical basis for calculating the mechanical properties of the glulam beam-column connection.