Four kinds of glued laminated timber were produced (i.e., one with a glued edge-joint and the other three with nonglued edge joints) in the lamina at different positions toward the depth direction. Shear tests using an asymmetric four-point bending method were then conducted for these glued laminated timber specimens. The results showed that although the glued edge-joint specimens had the highest shear strength in all groups, the shear strength decreased as the distance from the adjacent nonglued edge-joint plane decreased. Furthermore, the shear strength of all specimens exceeded the standard shear design strength value (2.1 N/mm2) set by the Ministry of Land, Infrastructure, Transport and Tourism, Japan. Next, the shear strength of the nonglued edge-joint specimens was estimated based on that of the glued edge-joint specimens. Although the mean-estimated shear strength was lower than the mean-measured shear strength, the possibility of the shear strength changing based on the position of the nonglued edge-joint plane specimens from that of the glued edge-joint specimens was still estimated.