This paper presents the results of long-term experiments performed on three timber-concrete composite (TCC) beams. An innovative fabricated steel plate connection system, which consists of screws and steel plates embedded in concrete slabs, was adopted in the TCC beam specimens. The adopted shear connection can provide dry-type connection for TCC beams. Steel plates were embedded in concrete slabs while the concrete slab was constructed in factories. The timber beam and concrete slab can be assembled together using screws at the construction site. In this experimental programme, the beam specimens were subjected to constant loading for 613 days in indoor uncontrolled environments. The influence of long-term loading levels and the number of shear connections on the long-term performance of TCC beams was investigated and discussed. The mid-span deflection, timber strain, and interface relative slip at the positions of both connections and beam-ends were recorded throughout the long-term tests. It was found the long-term deflection of the TCC beam increased by approximately 60% while the long-term loads were doubled. Under the influence of the variable temperature and humidity, the TCC specimens with 8 shear connections showed slighter fluctuations compared with the TCC beam with 6 shear connections. In the 613-day observation period, the maximum deflection increment recorded was 6.56 mm for the specimen with eight shear connections and 20% loading level. A rheological model consisting of two Kelvin bodies was employed to fit the curves of creep coefficients. The final deflections predicted of all specimens at the end of 50-year service life were 2.1~2.7 times the initial deflections caused by the applied loads. All beam specimens showed relative small increments in mid-span deflection, strain and relative slip over time without any degradations, demonstrating the excellent long-term performance of TCC beams using the innovative steel plate connection system, which is also easily fabricated.