In order to explore bamboo glulam utilization in structure construction, the adhesive bonded steel connection of bamboo glulam was investigated in this study. By carrying out both-end pullout tests on glued-in threaded rods in bamboo glulam, the effects of depth and diameter of embedded rods in bamboo glulam on the pullout strength and the failure modes were discussed. Results showed that threaded rods fracture and adhesive interface failure were the two main different failure modes in the tests. The pullout peak load of both-end glued-in rods in bamboo glulam increased with the diameter and the embedded length of the threaded rods. To satisfy tensile load of the glued threaded rods (quality 4.8) used in the connections between engineering structural materials, the slenderness ratio (the ratio of depth and diameter of glued-in threaded rods) equal to 10 or over was necessary.