This paper presents an experimental study on rolling shear (RS) strength properties of non-edge-glued cross-laminated timber (CLT) made out of New Zealand Radiata pine (Pinus radiata) structural timber. CLT specimens with 35 and 20 mm thick laminations were studied to evaluate the influence of lamination thickness on the RS strength of CLT. Short-span three-point bending tests were used to introduce high RS stresses in cross layers of CLT specimens and facilitate the RS failure mechanism. Modified planar shear tests from the conventional two-plate planar shear tests were also used to evaluate the RS strength properties. It was found that two test methods yielded comparable RS strength properties and the lamination thickness significantly affected RS strength of the CLT specimens. The test results also indicated that the recommended characteristic RS strength values of CLT products in Europe and Canada might be over conservative. Also, it might be more efficient to specify different RS strength values for CLT with different lamination thickness given the minimum width-to-depth ratio of laminations is satisfied.