Use of poplar (Populus euramericana cv. I-214) as cross layer to manufacture cross-laminated timber (CLT) was examined in this study. For comparison purpose, Douglas fir (Pseudotsuga menziesii) and Monterey pine (Pinus radiata D.Don) were used as well to produce five layups of CLT panels. The mechanical properties tested in this study included the bending strength in the major direction, modulus of elasticity in the major direction, shear strength parallel to the major direction and shear strength perpendicular to the major direction. It was found that the mechanical properties of CLT panels containing poplar were similar to those made of non-poplar wood. The major failure modes found were joint failure, shear failure and delamination. It could be feasible to use poplar as a cross layer to fabricate CLT without decreasing its strength properties.