This study aims to develop an improved understanding of the interfacial bond behavior of softwood glulam joints with bonded-in threaded steel rod. A total of 39 glulam joints with bonded-in single-threaded steel rods were tested to failure in the pull-pull configuration. The test results were presented in term of failure modes, load-relative movement response, pullout strength, and the corresponding slip. The distributions of bonded-in rod axial strain, interfacial bond stress, and relative movement were also analyzed to evaluate the local bond stress– relative movement response in the bond line. The results confirmed that the bond-relative movement response is dependent on the locations along the anchorage length, and the bond-relative movement responses located near both the loaded end and the anchorage end were observed to be stiffer than those at other locations. Finally, the predictions for the load capacity of the glulam joints with bonded-in threaded steel rod were carried out based on several existing empirical formulas.