The purpose of this research is to compare the bending behaviour of non-glue-laminated timber beams and glulams by full-scale four-point bending tests. The focus is on the non-glue beams laminated by different materials or techniques and then to determine their bending stiffness and failure modes. The laminating efficiency of various materials or techniques is underlined. The manufacturing process concerning non-glue-laminated timber beams has to be determined. As structural elements with large dimensions, such components require adaptable laminating and producing techniques. While the beams composed of wooden dowels refer to the dowel-laminated timber (DLT), those made of self-tapping screws (STSs) can be simply related to nail-laminated timber (NLT) products. Then, a full-scale four-point bending test was carried out to appraise 26 laminated beams, including non-glue- and glue-laminated timber. The results of the test demonstrated that the material, the spacing and the angle of the transversal fasteners significantly influence bending behaviour. The bending stiffness of the beams laminated by STSs was about 7.86% higher than the value of the beams with wooden dowels, although the tendency of each pair of beams did not remain convergent. Reducing the interval of the fasteners can considerably increase the bending stiffness of the beams. Fasteners inserted at 45 degrees, or in a so-called V-type pattern, contribute to improving bending stiffness, and both wooden dowels and STSs reveal the same tendency. At this angle, STSs demonstrate better laminating efficiency than wooden dowels. The STS beams’ bending stiffness was about 48.6% of that determined for glulams. On the contrary, in beams with 135-degree fasteners, or, namely, an A-type pattern, inserted fasteners possessed lower bending stiffness than in those with 90-degree fasteners. In addition to the considerable bending stiffness, the STS beams revealed a stable response as far as their load-deflection curves were concerned. A comparison of experimental and theoretical results contributes to verifying the feasibility as well as the weakness of two analytic methods. The predicting capacity of the associated equations needs to be improved, particularly for the withdrawal resistance and connecting effect of inclined STSs.