Design of mass timber floor systems is commonly governed by vibration serviceability due to high stiffness-to-weight ratio and low inherent damping of timber. Research and design practice have shown that static deflection under a concentrated load and fundamental natural frequency can be effective and robust indicators for vibration performance of mass timber floors. These design parameters are normally calculated by assuming simply supported conditions in existing design methods. However, such an assumption deviates from actual floor supports, especially in platform-framed buildings, and in-situ end support restraints have been widely recognized as a significant factor affecting the vibration performance. The purpose of the study is to quantify the influence of end support restraints on vibration serviceability of mass timber floors in platform construction through a comprehensive experimental program and analytical treatment. This paper is the first part and focus specifically on the experimental work on cross-laminated timber (CLT) panels. In particular, extensive laboratory tests have been conducted on different CLT floor panels with various end support restraints induced by top loads, self-tapping screws and steel angle brackets. The fundamental natural frequency and mid-span deflection under a concentrated load were measured for each end support configuration. The rotational restraint stiffness was determined by comparing results of restrained supports to those of simple supports and represented as end fixity factors. The analysis of test results shows that the CLT floor-to-wall connection exhibited inherent non-linear behaviour and such characteristic was more significant for higher top loads. Compared with screws and brackets, the top loads dominated the partially restrained effect but such dominance gradually diminished for lower-level top loads. In addition, support wall thickness notably impacts the support restraint. It was then suggested that the clear span could be used to determine deflection and frequency in the design, but further investigation is needed.