The in-plane shear performance of CLT using the asymmetric four-point bending test method was conducted to examine the test conditions. The parameters of specimens were the span, the height, the layups and the loading direction. As a result, it was found that the bending failure occurred when the span ratio was 1.25 times. This suggests that shear failure may occur reliably below the span ratio was 1.0 times. The shear strength was increased when loaded with the same lamina at the span ratio of less than 0.5 times. Therefore, it is recommended that the span ratio was 0.5 to 1.0 times to adapt for CLT in-plane shear testing method. The shear strength and the percentage of perpendicular layers were positively correlated, suggesting that shear strength may be estimated in a simplified manner. The diagonal measurement of shear modulus is considered to be the easier way to calculate the shear modulus, as it does not require any concern for the grain direction. The shear modulus values ranged from half to four times higher than the design values in Japan. A comparison of the shear strength and shear modulus between values in the reference using other testing methods and the experimental values in this study shows a tendency to show different values. This may be due to the different shear section areas of the specimens depending on the test method and the different restraint conditions.