The design of timber-concrete composite structures (TCC) is usually governed by the serviceability rather than the strength, particularly for medium to long span floors.In terms of users’ comfort, issues can come from vibrations of TCC floors. Indeed, discrepancies are usually observed between the calculations at the design stage and the on-site measurements, due to the gap between design assumptions and in service conditions (boundary conditions, partition walls, floor finishing…). This paper focuses on the comparison between analytical, experimental (on-site and laboratory) and numerical approaches in the assessment of vibration performance of a TCC floor to obtain the frequencies corresponding to different boundary conditions. Results show unexpected differences between analytical models and laboratory experimental measurements for simple boundary conditions. A new equation is being developed to adapt the design of the TCC floors of the full-scale building under study, which presents the specificity of a TCC floor with overhang. A FEM model is developed to assess the validity of the proposed analytical equation.