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In massive production, mass timber sections made of engineered wood, such as cross-laminated timber (CLT) panels, accelerated the pace of the construction of tall wood buildings in North America in the past few years. However, more stringent fire safety measures are applicable to tall timber buildings. Most critically, floor systems in mass timber buildings can be made more robust, span longer distances and exhibit enhanced fire resistance by adding a top layer of concrete to allow a timber-concrete composite (TCC) floor system when adequate shear connections are utilized. This paper presents the experimental results of the fire resistance tests of two full-size, one-way TCC floor slabs that utilized self-tapping screws (STS) as shear connectors. The main objective of this new experimental study is to investigate the enhancement of the fire performance when designing a CLT panel as a TCC floor assembly through achieving the required composite action with a top concrete layer at the interface between the two materials. Test results show that a minimum fire resistance of two hours required by applicable building codes for mid-rise residential buildings has been achieved for CLT floor panel by designing it as a TCC floor assembly, taking advantage of the composite action activated by the utilization of 45-degree inclined STS as shear connectors.