The development of sustainable and efficient building solutions has become imperative in the face of depleting construction resources and increasing demand. This is providing a boost for timberbased construction and prefabricated systems. In this research, a flooring solution having attributes that address these needs is studied: a prefabricated modular CLT-steel hybrid floor assembly. The floor features an efficient composite system, lightweight components which enhance the ease of installation and a completely dry assembly process. It also provides the possibility for end-of-life disassembly and repurposing of its components. However, a detailed understanding of its structural behaviour and design is crucial to its uptake and implementation in the building industry. One significant area of knowledge gap is its vibration characteristics and serviceability performance when subjected to human-induced activities. These are often dominant in the design of lightweight systems like the one under consideration and hence they are in focus in this study. In the second phase of the project, which is presented in this report, vibration tests and subjective vibration performance evaluations were undertaken. The results indicate that the floor is a high-frequency system and can attain adequate levels of vibration comfort in residential and office settings. The vibration dose value method was also found to be suitable for predicting its vibration performance when its modules are connected via self-tapping screws.