Nail-laminated timber (NLT) can be used as structural floors to transfer lateral force.However,the research onthe in-plane performance of NLT floorsisstill insufficient. This paper presents the results of an experimental investigation on the in-plane performance of NLT floors. ThreeNLT floorconfigurations, a total of ninespecimens, considering different sheathing nail spacings and orient strand board (OSB) sheathing fibreorientations, were subjected to fully reversed cyclic loading.Sheathing nail and laminanail failure were observed as main failure mode during the cyclic loading. Sheathing orientation was noticed to have impact on theNLTfloor deformation pattern. Equivalent energy elastic-plastic curveswereobtainedfromeach specimengroup, from whichthe elastic shear stiffness, yielding load and displacement, ductility ratio, and shear strengthwere calculated.Secant stiffness and dissipated energy were also extracted from hysteresis loops.The comparison indicates that arranging OSBfibreperpendicular to laminaaxis could significantlyincreasethe elastic shear stiffness and ductility ratio of NLT floor, and a tightened sheathingnail spacing(i.e., 75and 150 mm at the edge and centre, respectively)could provide higher elastic shear stiffness, shear strength, ductility, and improve energy dissipation capacity.