To evaluate the static and seismic behaviour of glulam beam-to-column connections with screwed-in threaded rods, nine specimens grouped in three were tested under both monotonic and reversed cyclic loads. The failure modes, moment resistance, initial rotation stiffness, ductility, and energy dissipation capacity of the developed connections were investigated. The results indicated that the developed beam-to-column connections showed superior structural performance. Furthermore, with the introduction of a steel bracket, the hybrid screwed-in threaded rod connection features larger stiffness, higher load-carrying capacity, remarkable ductility, and better energy dissipation capacity. The main failure modes included the yielding of steel brackets, as well as the yielding or rupture of the threaded rods, which indicated a ductile behaviour. The connection specimens with steel columns showed larger stiffness than those with glulam columns, which is reasonable for the bigger compressive deformation of glulam columns.