Cross-laminated timber (CLT), a wood product with excellent shear resistance, is often used in modern timber constructions. Using the standards ASTM D1761-12 (2020) and NDS-2012 (2012), this study investigated the connection properties of shear bolts and screws in CLT panels. The specimens were made from spruce-pine-fir lumber and installed on a test platform using one high-strength bolt or eight screws, and then an upward load was applied to the top of the specimen. The results showed that the bolt connection provided a higher ultimate bearing capacity and elastic stiffness. The bolt exhibited virtually no deformation, and the CLT panel did not noticeably deteriorate when the connection was damaged. The distance between the bolt hole and the bottom of the CLT specimen and the angle between the outer-layer grain direction of the CLT panel and the load direction were both measured. Changes in the ductility coefficient value had an obvious effect on the connection performance of the shear bolts when the outer-layer grain direction of the CLT panel was consistent with the load direction. Contrastingly, when the outer-layer grain direction of the CLT panel was perpendicular to the load direction, the effect was negligible, and the yield load was nearly unchanged.