Timber structures are currently more important for solving tasks in construction practice. For this reason, there is an opportunity for research in the area of physical tests and numerical models. This paper deals with the determination and comparison of the deformation properties of cross-laminated timber (CLT) panels based on laboratory tests, analytical calculation and numerical modeling. CLT panels are structural building components consisting of cross-oriented solid timber layers. Three types of panels with different geometry and number of layers (three, five and seven) were experimentally tested using a four-point bending test, where load–deformation curves were recorded. The results of the experimental testing of the three-layer panels were subsequently compared with a numerical model in SCIA Engineer, a numerical model in ANSYS Workbench and an analytical calculation. The research shows a good agreement in bending behavior between the laboratory tests, the analytical calculation according to the standard and two different approaches in numerical analysis.