Design for disassembly using deconstructable connections facilitates recycling and reusing of building materials and, therefore, reduces waste management problems at the end of service life. In this regard, a deconstructable timber-concrete composite connector using self-tapping screws has been developed at Aalto University. In the presented research, an experimental investigation was performed to further evaluate the effectiveness of this connector in fabricating deconstructable cross-laminated timber (CLT)-concrete composite floors. For this purpose, several CLT-concrete composite beams were fabricated using 5-layer CLT and low-shrinkage concrete. Each beam contained one row of connectors to represent a strip of a full-scale floor. The vibration performance, bending properties, interface slip, failure modes, and ease of disassembly of the beams were investigated. The results were compared with the ones from a reference group of beams fabricated with regular screws. Overall, the deconstructable beams performed exceptionally well by attaining 98.5% of the average bending stiffness of the regular beams. The load-carrying capacity was also similar but governed by the CLT plate. The vibration characteristics were comparable in both groups. After the bending test, the deconstructable beams were disassembled. Although the beams had been exposed to unproportionally large deformations under the bending load, the disassembly process was performed successfully.