This study aimed to investigate the effect of layer arrangement on bending properties of CLT panels made from poplar (Populus deltoides L.). A total of 20 three-layer CLT panels with the same dimensions of 1300 × 360 × 48 mm3 (Length, Width, Thickness) were fabricated in five configurations: 0/30/0, 0/45/0, 0/90/0, 45/0/45, and 45/45/45. The apparent modulus of elasticity (MOEapp), modulus of rupture (MOR) and apparent bending stiffness (EIapp) values in major and minor axes of CLT panels were calculated using experimental bending testing. In the major axis, the highest values of MOR, MOEapp, and EIapp were obtained from the 0/30/0 arrangement, while the least values resulted from the arrangements of 90/60/90 and 90/45/90 in the minor axis. Besides, in all arrangements, the average of the experimental apparent bending stiffness values (EIapp,exp) of specimens was higher than that of the shear analogy apparent bending stiffness values (EIapp,shear). The bending and shear stress distribution values over the cross section of samples were also estimated using the finite element method. Moreover, the numerical apparent bending stiffness (EIapp,fem) values of samples were compared to experimental apparent bending stiffness (EIapp,exp) values. Based on experimental and finite element method results, in all groups of layer arrangements, the EIapp,fem values concurred well with the EIapp,exp values.