Cross-Laminated Timber (CLT) is a building technology that is becoming increasingly popular due to its sustainable and eco-friendly nature, as well as its availability. Nevertheless, CLT presents some challenges, especially in terms of impact noise and airborne sound insulation. For this reason, many studies focus on the vibro-acoustic behavior of CLT building elements, to understand their performance, advantages and limitations. In this paper, a 200 mm CLT floor has been characterized in the laboratory, according to ISO standards, by three noise sources: dodecahedron, standard tapping machine and rubber ball. In order to understand the vibro-acoustic behavior of the CLT floor, measurements through the analysis of sound pressure levels and velocity levels, measured by dedicated sensors, were performed. Analysis was carried out in order to understand what is prescribed by the prediction methods available in the literature and by the simulation software. Then, a specific prediction law for the CLT floor under investigation was derived. Finally, an analysis on sound radiation index is provided to complete the vibro-acoustic study.