The feasibility of a portable NIR sensor for off-line determination of diverse wood quality aspects relevant in the production of glue-laminated timber was demonstrated. The best performance was noticed for assessing wood moisture content, with a lower capacity to estimate wood density and mechanical properties. NIR spectroscopy was modestly capable of predicting surface roughness. However, the traceability of the raw resources and the automatic classification of diverse wood defects were successfully demonstrated. The developed chemometric model could predict the total delamination and detailed delamination length. Finally, recommendations regarding further system development were provided with the aim of implementation and integration of the NIR measurement into glue-laminated timber production plants.