This paper deals with the influence of the rolling shear deformation on the flexural behavior of CLT (Cross-Laminated Timber) panels. The morphological configuration of the panels, which consist of orthogonal overlapped layers of boards, led to a particular shear behavior when subjected to out-of-plane loadings: the low value of the shear modulus in orthogonal to grain direction (i.e., rolling shear modulus) gives rise to significant shear deformations in the transverse layers of boards, whose grains direction is perpendicular with respect to the tangential stresses direction. This produces increases of deflections and vibrations under service loads, creating discomfort for the users. Different analytical methods accounting for this phenomenon have been already developed and presented in literature. Comparative analyses among the results provided by some of these methods have been carried out in the present paper and the influence of the rolling shear deformations, with reference to different span-to-depth (L/H) ratios investigated. Moreover, the analytical results have also been compared with those obtained by more accurate 2D finite element models. The results show that, at the service limit states, the influence of the rolling shear can be significant when the aspect ratios became less than L/H = 30, and the phenomenon must be accurately considered in both deflection and stress analysis of CLT floors. Contrariwise, in the case of higher aspect ratios (slender panels), the deflections and stresses can be evaluated neglecting the rolling shear influence, assuming the layers of boards as fully-connected.