Long-span timber-concrete composite (TCC) floor systems have the potential to address the design challenges for conventional wooden floors in residential multi-storey timber frame buildings. The aim of this paper is to develop a design approach for long-span timber-concrete composite floor system of 6–9 m. A framework based on value-driven design approach has been developed for integration of results from graphical multi-objective optimisation, spreadsheet-based analysis, structural static and dynamic finite element analysis, and multi-criteria decision making. To verify the developed framework, a residential five-storey timber frame building as a case study has been studied. Optimal design includes optimised thickness of the concrete and optimised smeared stiffness of connectors for three different comfort classes A to C in descending order. TCC floor with span length 7.3 [m] belonging to comfort class A and TCC floor with span length 9.0 [m] belonging to comfort class C has been chosen as optimal solutions. The results indicate that proposed and innovative design approach is a promising tool for developers, architects and structural engineers when designing optimal long-span timber-concrete composite floor system.