Through long-term measurements of climate data (temperature, relative humidity) and timber moisture content on large-span timber structures in buildings of typical construction type and use, data sets were generated which deliver information on the sequence and magnitude of seasonal variations. The measurement of moisture in different depths of the cross-section is of particular interest to draw conclusions on the size and speed of adjustment of the moisture distribution to changing climatic conditions. The moisture gradient has direct influence on the size of the internal stresses and possible damage potential. Similarly, the results provide a review and extension of the previous classification of buildings into use classes. They allow for a more precise indication of range of resulting equilibrium moisture content for the specific use, enabling the installation of timber elements with adjusted moisture content. The results of the research project also support the development of appropriate monitoring systems, which could be used in the form of early warning systems based on climate measurements.