Cross-laminated timber (CLT) is a wood panel product that can be arranged in different ways. The advantage of utilizing CLT is the ability to use lamination even with low density materials or those that have defects, like knots. This study evaluated the bonding and bending performances of CLT utilizing domestic species in a shear wall or floor via a face bonding test of layers and a three-point bending test. The tests were carried out with three-layered CLT made up of Japanese larch and/or Korean red pine in various configurations. The layer arrangement for lamination was divided according to the species and grade of the wood. The out-of-plane and in-plane bending tests were conducted on the CLT according to the applicable direction in a wooden structure. The results of the bonding test showed that the block shear strength and delamination of all types of CLT met the BS EN 16351 (2015) standard requirements. The results of the bending test based on two wood species showed that the bending strength of the larch CLT was higher than that of the pine CLT in single species combinations. For mixed species combinations, the bending properties of CLT using larch as the major layer was higher than those using pine as the major layer. This demonstrated that the major layer had more influence on the bending properties of CLT and that Korean red pine was more suited for the minor layer of CLT.