The purpose of this study is to examine the mechanical properties of ACQ-treated glulam made from three hardwood lumbers. Two nondestructive methods, ultrasonic wave and tap tone method, were also used in this study. The results showed that the dynamic MOE and static MOE of lumbers decreased with increased ACQ preservative retention. ANOVA showed no significant difference in the MOE values of glulam between untreated and ACQ-treated group. However, it was also found that glulam made from red oak lumbers had the highest bending strength retention ratio. The shear strength of the glulam also showed similar results. Finally, no delaminating was found in all glulams after the specimens under soaked and boiled delamination tests.