This research study investigates the effect of micronized copper azole type C (MCA-C) preservative system on the rolling shear (RS) properties of CLT. In the first part of research, bonding performance of CLT panels treated at two retention levels, 0.96 kg/m3 and 2.5 kg/m3, were evaluated. Three structural adhesive systems, melamine formaldehyde (MF), resorcinol formaldehyde (RF) and one-component polyurethane (1C-PUR) were used to assemble visually graded No. 2 2×6 southern yellow pine (SYP) lumber while manufacturing CLT panels. For treated CLT panels, 1C-PUR provides better bonding performance test results. The RS properties of MCA-C treated CLT panels were studied in the second part of the research. The CLT panels were subjected to out-of-plane loading according to the EN 16351 standard. The mean values of RS strength and modulus of treated CLT were 1.89 MPa and 289.4 MPa respectively.