The objective of this research was to determine the feasibility of using lumber from Northeastern U.S. forests and laminated strand lumber (LSL) in cross laminated timber (CLT). Three-layer 105 mm x 1.32 m x 2.45 m CLT panels consisting solely of Spruce-Pine-Fir (South) and 1.35E LSL were manufactured by hand. Four-point bending tests were performed for specimens in the minor and major strength directions. From these tests, bending strengths and elastic and shear stiffnesses were determined for both strength directions. The all-SPFs lay-up exceeded the design requirements of CLT grade E1 and the all-LSL lay-up exceeded those of CLT grade E3. Therefore, low grade SPFs could be used to create high grade CLT panels. In addition, the use of LSL as the cross-ply material in CLT could increase the perpendicular-to-grain shear strength of CLT designs in comparison to typical lumber, which can enhance overall panel stiffness and strength.