Japanese larch (Larix kaempferi) is an important species in China. The extensive use of Japanese larch can alleviate lumber shortage in China. Various grades of Japanese larch (Larix kaempferi) laminas were chosen to study the factors that affect the bolted joint performance. By comparing the loading test results with the required design values,it revealed that: (1) Bolt diameter affected the loading performance, then, bolt class, and the lamina grade was the least influential factor. (2) Japanese larch glulam can achieve the required designed value to be used as structural materials.